PEO-302,过氧化物酶（Peroxidase）1000KU-TOYOBO生物试剂

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PEO-302 - 过氧化物酶（Peroxidase）

过氧化物酶（Peroxidase）

商品品牌： TOYOBO
分类：诊断试剂及研究
Spec： 1000KU
货期：咨询

询价

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PREPARATION and SPECIFICATION

PEO-301/302 are isolated from horseradish root in our own method. PEO-131 is chromatographically separated from the other isoenzymes using SP Sephadex C-50 by the modified method of Paul et al.¹⁾ The peroxidase fractions having equal RZ values (ca.3.3) are combined to generate the preparation. This preparation has an RZ value of ca.3.3 and is electrophoretically homogeneous. On the other hand, GradeIII is partially purified preparation.

Appearance	Reddish-brown amorphous powder, lyophilized
Activity	GradeⅠ　250 Purpurogallin U/mg-solid or more (-131) (RZ ≥ 3.0, salt free) GradeⅢ　110 Purpurogallin U/mg-solid or more (-301) (RZ ≥ 2.0, containing approx. 30 % of stabilizers) GradeⅢ　180 Purpurogallin U/mg-solid or more (-302) (RZ ≥ 2.0, salt free)
Contaminant	Phosphatase ≤1.0×10-3 % (GradeIII)

PROPERTIES

Stability	Stable at −20 ℃ for at least one year（Fig.1,2,3）
Molecular weight	approx. 40,000
Structure	Glycoprotein with one mole of protohaeminIX ^2）
Inhibitors	Cyanide, sulfide, fluoride, azide ³）
Optimum pH	6.0−7.0（Fig.6）
Optimum temperature	45 ℃（Fig.7）
pH Stability	pH 5.0−10.0 (25 ℃, 20 hr)（Fig.8）
Thermal stability	below 50 ℃ (pH 6.0, 10 min)（Fig.9）
Effect of various chemicals	(Table 1)

APPLICATIONS4〜11)

This enzyme is useful for enzymatic determination of H2O2 in clinical analysis. In particular, the highly purified preparation (grade I) is used as a protein-tracer in histo and cyto-chemistry and as a valuable experimental tool in hodological neurography. In addition, the enzyme preparation has been used as an enzyme label in enzyme immunoassays. Grade III(-302) is suitable for dry chemistry. Furthermore, the enzyme contributes to reduction of phehol in waste water.

ENZYMES : DIAGNOSTIC REAGENT GRADE

DESCRIPTION	CODE	ORIGIN	GRADE	ACTIVITY	SDS
N-Acetylneuraminic acid aldolase	NAL-301 *2	Microorganism	III	15 U/mg-solid or more	↓SDS
Alkaline phosphatase	LPP-229 *1	Microorganism	II	30,000 U/ml or more
Ascorbate oxidase	ASO-301	Cucumis sp.	III	200 U/mg-solid or more	↓SDS
Ascorbate oxidase	ASO-311	Cucurbita sp.	III	200 U/mg-solid or more	↓SDS
Catalase	CAO-519	Microorganism	V	150,000 U/ml or more	↓SDS
Cholesterol esterase	COE-301	Schizophyllum commune	III	2.0 U/mg-solid or more	↓SDS
	COE-302	Schizophyllum commune	III	2.0 U/mg-solid or more
	COE-311	Pseudomonas sp.	III	100 U/mg-solid or more	↓SDS
	COE-313	Microorganism	III	5.0 U/mg-solid or more	↓SDS
Cholesterol oxidase	COO-321	Microorganism	III	12 U/mg-solid or more	↓SDS
Cholesterol oxidase	COO-331	Microorganism	III	12 U/mg-solid or more
Choline oxidase	CHO-301	Alcaligenes sp.	III	10 U/mg-solid or more	↓SDS
Creatine amidinohydrolase	CRH-211	Actinobacillus sp.	II	6.0 U/mg-solid or more	↓SDS
	CRH-221	Microorganism	II	4.0 U/mg-solid or more	↓SDS
	CRH-229	Microorganism	II	2,000 U/ml or more
Creatinine amidohydrolase	CNH-211	Pseudomonas sp.	II	450 U/mg-solid or more
Creatinine amidohydrolase	CNH-311	Pseudomonas sp.	III	150 U/mg-solid or more	↓SDS
Creatinine deiminase	CNI-311	Microorganism	III	10 U/mg-solid or more	↓SDS
Diaphorase	DAD-301	Clostridium sp.	III	30 U/mg-solid or more	↓SDS
Diaphorase	DAD-311	Microorganism	III	500 U/mg-solid or more	↓SDS
β-Galactosidase	GAH-201	Escherichia coli	II	500 U/mg-solid or more	↓SDS
Glucoamylase	GLA-111	Rhizopus sp.	I	30 U/mg-solid or more	↓SDS
Glucose dehydrogenase(NAD(P)-dependent)	GLD-311	Microorganism	III	250 U/mg-solid or more	↓SDS
Glucose dehydrogenase(PQQ-dependent)	GLD-321	Microorganism	III	500 U/mg-solid or more	↓SDS
Glucose oxidase	GLO-101	Aspergillus sp.	I	180 U/mg-solid or more	↓SDS
Glucose oxidase	GLO-201	Aspergillus sp.	II	100 U/mg-solid or more	↓SDS
Glucose-6-phosphate dehydrogenase	G6D-311	Leuconostoc sp.	III	400 U/mg-solid or more	↓SDS
Glucose-6-phosphate dehydrogenase	G6D-321	Microorganism	III	200 U/mg-solid or more	↓SDS
α-Glucosidase	AGH-211	Microorganism	II	20 U/mg-solid or more	↓SDS
β-Glucosidase	BGH-101	Sweet almond	I	15 U/mg-solid or more	↓SDS
Glutamate dehydrogenase(NADP dependent)	GTD-209	Proteus sp.	II	300 U/mg-protein or more	↓SDS
Glutamate dehydrogenase(NADP dependent)	GTD-309	Proteus sp.	III	300 U/mg-protein or more	↓SDS
Glutamate dehydrogenase(NAD dependent)	GTD-211	Microorganism	II	100 U/mg-solid or more	↓SDS
Glycerol dehydrogenase	GYD-301	Cellulomonas sp.	III	50 U/mg-solid or more	↓SDS
Glycerol kinase	GYK-301	Cellulomonas sp.	III	20 U/mg-solid or more	↓SDS
Glycerol kinase	GYK-311	Microorganism	III	30 U/mg-solid or more	↓SDS
L-α-Glycerophosphate oxidase	G3O-321	Microorganism	III	15 U/mg-solid or more	↓SDS
Hexokinase	HXK-311	Microorganism	III	150 U/mg-solid or more	↓SDS
p-Hydroxybenzoate hydroxylase	HBH-311	Microorganism	III	20 U/mg-solid or more	↓SDS
D-3-Hydroxybutyrate dehydrogenase	HBD-301	Achromobacter sp.	III	100 U/mg-solid or more	↓SDS
D-3-Hydroxybutyrate dehydrogenase	HBD-311	Achromobacter sp.	III	450 U/mg-solid or more
Invertase	IVH-101	Candida sp.	I	100 U/mg-solid or more	↓SDS
D-Lactate dehydrogenase	LCD-211	Microorganism	II	400 U/mg-solid or more	↓SDS
D-Lactate dehydrogenase	LCD-221	Microorganism	II	400 U/mg-solid or more	↓SDS
Lactate Oxidase	LCO-301	Microorganism	III	80 U/mg-solid or more	↓SDS
Leucine dehydrogenase	LED-201	Bacillus sp.	II	20 U/mg-solid or more
Lipoprotein lipase	LPL-311	Pseudomonas sp.	III	20 U/mg-solid or more	↓SDS
Lipoprotein lipase	LPL-314	Microorganism	III	1.0 U/mg-solid or more	↓SDS
Malate dehydrogenase	MAD-211	Microorganism	II	40 U/mg-solid or more	↓SDS
Peroxidase	PEO-131	Horseradish	I	250 PU (RZ ≥ 3.0)	↓SDS
	PEO-301	Horseradish	III	110 PU (RZ ≥ 2.0)	↓SDS
	PEO-302	Horseradish	III	180 PU (RZ ≥ 2.0)	↓SDS
	PEO-701 *1	Horseradish	VII	70 PU (RZ ≥ 0.6)
Phosphoenolpyruvate carboxylase	PPC-301	Microorganism	III	5.0 U/mg-solid or more	↓SDS
Proline specific endopeptidase	PSP-101	Flavobacterium sp.	I	5.0 U/mg-solid or more	↓SDS
Protocatechuate 3,4-dioxygenase	PCO-302	Pseudomonas sp.	III	3.0 U/mg-solid or more	↓SDS
Pyruvate oxidase	PYO-311	Pseudomonas sp.	III	1.5 U/mg-solid or more	↓SDS
Purine-nucleoside phosphorylase	PNP-311	Microorganism	III	15 U/mg-solid or more	↓SDS
Sarcosine oxidase	SAO-351	Microorganism	III	8.0 U/mg-solid or more	↓SDS
Superoxide dismutase	SOD-302	Bovine erythrocyte	III	3,000 U/mg-solid or more	↓SDS
Urease	URH-201	Jack bean	II	100 U/mg-solid or more	↓SDS
Uricase	UAO-201	Candida sp.	II	4.0 U/mg-solid or more	↓SDS
Uricase	UAO-211	Microorganism	II	1.5 U/mg-solid or more	↓SDS
Xanthine oxidase	XTO-212	Microorganism	II	10 U/mg-solid or more	↓SDS

ENZYMES : INDUSTRIAL GRADE

DESCRIPTION	CODE	ORIGIN	GRADE	ACTIVITY
N-Acetylneuraminic acid aldolase	NAL-301 *2	Microorganism	III	15 U/mg-solid or more

SUBSTRATE : DIAGNOSTIC REAGENT GRADE

NAL-301

DESCRIPTION	CODE	ORIGIN	GRADE	ACTIVITY	SDS
Gal-G2-α-CNP	CNP-005	Amylase substrates			↓SDS

BIOCHEMICAL PRODUCTS : DIAGNOSTIC REAGENT GRADE

DESCRIPTION	CODE	ORIGIN	GRADE	ACTIVITY	SDS
Blocking Peptide Fragment	BPF-301	Microorganism	III		↓SDS
Blocking Peptide Fragment	BPF-302	Microorganism	III
Neo Protein Saver	NPS-301 *2	Silk Protein	III		↓SDS

*1 Under development. Sample available. Please contact us.

*2 To be discontinued. Available only while stock lasts.

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