LCO-301-5,乳酸氧化酶5ku-TOYOBO诊断试剂及研究

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LCO-301-5 - 乳酸氧化酶

乳酸氧化酶

商品品牌： TOYOBO
分类：诊断试剂及研究
Spec： 5ku
货期：咨询

询价

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PREPARATION and SPECIFICATION

Appearance	Yellowish amorphous powder, lyophilized
Activity	GradeⅢ　80 U/mg-solid or more
Contaminants	Pyruvate oxidase	≤1.0×10^-3 %
	Cholesterol oxidase	≤1.0×10^-3 %
	Uricase	≤1.0×10^-3 %
	Glucose oxidase	≤1.0×10^-3 %

PROPERTIES

Stability	Stable at −20 ℃ for at least one year(Fig.1)
Molecular weight	approx. 160,000 (by gel filtration)
Isoelectric point	4.3±0.2
Michaelis constant	1.0×10^-3 M (L-Lactate)
Inhibitors	Fe³⁺, SDS
Optimum pH	7.5(Fig.2)
Optimum temperature	35−40 ℃(Fig.3)
pH Stability	4.0−9.8 (25 ℃, 16 hr)(Fig.4)
Thermal stability	below 50 ℃ (pH 7.0, 10 min)(Fig.5)
Effect of various chemicals	(Table 1)

APPLICATIONS

This enzyme is useful for enzymatic determination of L-lactate

ASSAY

Principle

The formation of quinoneimine dye is measured at 555 nm by spectrophotometry.

ENZYMES : DIAGNOSTIC REAGENT GRADE

DESCRIPTION	CODE	ORIGIN	GRADE	ACTIVITY	SDS
N-Acetylneuraminic acid aldolase	NAL-301 *2	Microorganism	III	15 U/mg-solid or more	↓SDS
Alkaline phosphatase	LPP-229 *1	Microorganism	II	30,000 U/ml or more
Ascorbate oxidase	ASO-301	Cucumis sp.	III	200 U/mg-solid or more	↓SDS
Ascorbate oxidase	ASO-311	Cucurbita sp.	III	200 U/mg-solid or more	↓SDS
Catalase	CAO-519	Microorganism	V	150,000 U/ml or more	↓SDS
Cholesterol esterase	COE-301	Schizophyllum commune	III	2.0 U/mg-solid or more	↓SDS
	COE-302	Schizophyllum commune	III	2.0 U/mg-solid or more
	COE-311	Pseudomonas sp.	III	100 U/mg-solid or more	↓SDS
	COE-313	Microorganism	III	5.0 U/mg-solid or more	↓SDS
Cholesterol oxidase	COO-321	Microorganism	III	12 U/mg-solid or more	↓SDS
Cholesterol oxidase	COO-331	Microorganism	III	12 U/mg-solid or more
Choline oxidase	CHO-301	Alcaligenes sp.	III	10 U/mg-solid or more	↓SDS
Creatine amidinohydrolase	CRH-211	Actinobacillus sp.	II	6.0 U/mg-solid or more	↓SDS
	CRH-221	Microorganism	II	4.0 U/mg-solid or more	↓SDS
	CRH-229	Microorganism	II	2,000 U/ml or more
Creatinine amidohydrolase	CNH-211	Pseudomonas sp.	II	450 U/mg-solid or more
Creatinine amidohydrolase	CNH-311	Pseudomonas sp.	III	150 U/mg-solid or more	↓SDS
Creatinine deiminase	CNI-311	Microorganism	III	10 U/mg-solid or more	↓SDS
Diaphorase	DAD-301	Clostridium sp.	III	30 U/mg-solid or more	↓SDS
Diaphorase	DAD-311	Microorganism	III	500 U/mg-solid or more	↓SDS
β-Galactosidase	GAH-201	Escherichia coli	II	500 U/mg-solid or more	↓SDS
Glucoamylase	GLA-111	Rhizopus sp.	I	30 U/mg-solid or more	↓SDS
Glucose dehydrogenase(NAD(P)-dependent)	GLD-311	Microorganism	III	250 U/mg-solid or more	↓SDS
Glucose dehydrogenase(PQQ-dependent)	GLD-321	Microorganism	III	500 U/mg-solid or more	↓SDS
Glucose oxidase	GLO-101	Aspergillus sp.	I	180 U/mg-solid or more	↓SDS
Glucose oxidase	GLO-201	Aspergillus sp.	II	100 U/mg-solid or more	↓SDS
Glucose-6-phosphate dehydrogenase	G6D-311	Leuconostoc sp.	III	400 U/mg-solid or more	↓SDS
Glucose-6-phosphate dehydrogenase	G6D-321	Microorganism	III	200 U/mg-solid or more	↓SDS
α-Glucosidase	AGH-211	Microorganism	II	20 U/mg-solid or more	↓SDS
β-Glucosidase	BGH-101	Sweet almond	I	15 U/mg-solid or more	↓SDS
Glutamate dehydrogenase(NADP dependent)	GTD-209	Proteus sp.	II	300 U/mg-protein or more	↓SDS
Glutamate dehydrogenase(NADP dependent)	GTD-309	Proteus sp.	III	300 U/mg-protein or more	↓SDS
Glutamate dehydrogenase(NAD dependent)	GTD-211	Microorganism	II	100 U/mg-solid or more	↓SDS
Glycerol dehydrogenase	GYD-301	Cellulomonas sp.	III	50 U/mg-solid or more	↓SDS
Glycerol kinase	GYK-301	Cellulomonas sp.	III	20 U/mg-solid or more	↓SDS
Glycerol kinase	GYK-311	Microorganism	III	30 U/mg-solid or more	↓SDS
L-α-Glycerophosphate oxidase	G3O-321	Microorganism	III	15 U/mg-solid or more	↓SDS
Hexokinase	HXK-311	Microorganism	III	150 U/mg-solid or more	↓SDS
p-Hydroxybenzoate hydroxylase	HBH-311	Microorganism	III	20 U/mg-solid or more	↓SDS
D-3-Hydroxybutyrate dehydrogenase	HBD-301	Achromobacter sp.	III	100 U/mg-solid or more	↓SDS
D-3-Hydroxybutyrate dehydrogenase	HBD-311	Achromobacter sp.	III	450 U/mg-solid or more
Invertase	IVH-101	Candida sp.	I	100 U/mg-solid or more	↓SDS
D-Lactate dehydrogenase	LCD-211	Microorganism	II	400 U/mg-solid or more	↓SDS
D-Lactate dehydrogenase	LCD-221	Microorganism	II	400 U/mg-solid or more	↓SDS
Lactate Oxidase	LCO-301	Microorganism	III	80 U/mg-solid or more	↓SDS
Leucine dehydrogenase	LED-201	Bacillus sp.	II	20 U/mg-solid or more
Lipoprotein lipase	LPL-311	Pseudomonas sp.	III	20 U/mg-solid or more	↓SDS
Lipoprotein lipase	LPL-314	Microorganism	III	1.0 U/mg-solid or more	↓SDS
Malate dehydrogenase	MAD-211	Microorganism	II	40 U/mg-solid or more	↓SDS
Peroxidase	PEO-131	Horseradish	I	250 PU (RZ ≥ 3.0)	↓SDS
	PEO-301	Horseradish	III	110 PU (RZ ≥ 2.0)	↓SDS
	PEO-302	Horseradish	III	180 PU (RZ ≥ 2.0)	↓SDS
	PEO-701 *1	Horseradish	VII	70 PU (RZ ≥ 0.6)
Phosphoenolpyruvate carboxylase	PPC-301	Microorganism	III	5.0 U/mg-solid or more	↓SDS
Proline specific endopeptidase	PSP-101	Flavobacterium sp.	I	5.0 U/mg-solid or more	↓SDS
Protocatechuate 3,4-dioxygenase	PCO-302	Pseudomonas sp.	III	3.0 U/mg-solid or more	↓SDS
Pyruvate oxidase	PYO-311	Pseudomonas sp.	III	1.5 U/mg-solid or more	↓SDS
Purine-nucleoside phosphorylase	PNP-311	Microorganism	III	15 U/mg-solid or more	↓SDS
Sarcosine oxidase	SAO-351	Microorganism	III	8.0 U/mg-solid or more	↓SDS
Superoxide dismutase	SOD-302	Bovine erythrocyte	III	3,000 U/mg-solid or more	↓SDS
Urease	URH-201	Jack bean	II	100 U/mg-solid or more	↓SDS
Uricase	UAO-201	Candida sp.	II	4.0 U/mg-solid or more	↓SDS
Uricase	UAO-211	Microorganism	II	1.5 U/mg-solid or more	↓SDS
Xanthine oxidase	XTO-212	Microorganism	II	10 U/mg-solid or more	↓SDS

ENZYMES : INDUSTRIAL GRADE

DESCRIPTION	CODE	ORIGIN	GRADE	ACTIVITY
N-Acetylneuraminic acid aldolase	NAL-301 *2	Microorganism	III	15 U/mg-solid or more

SUBSTRATE : DIAGNOSTIC REAGENT GRADE

NAL-301

DESCRIPTION	CODE	ORIGIN	GRADE	ACTIVITY	SDS
Gal-G2-α-CNP	CNP-005	Amylase substrates			↓SDS

BIOCHEMICAL PRODUCTS : DIAGNOSTIC REAGENT GRADE

DESCRIPTION	CODE	ORIGIN	GRADE	ACTIVITY	SDS
Blocking Peptide Fragment	BPF-301	Microorganism	III		↓SDS
Blocking Peptide Fragment	BPF-302	Microorganism	III
Neo Protein Saver	NPS-301 *2	Silk Protein	III		↓SDS

*1 Under development. Sample available. Please contact us.

*2 To be discontinued. Available only while stock lasts.

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